ACU may refer to:
- Educational institutions
- Abilene Christian University, a private Christian university located in Abilene, Texas
- Ahram Candian University, a private Egyptian university located in 6 October City, Giza, Egypt
- American City University in California or Wyoming
- Association of Commonwealth Universities, an association that represents over 480 universities from Commonwealth countries
- Australian Catholic University, Australia's only public Catholic university
- Ajayi Crowther University,a private Christian university located in Oyo, Oyo State Nigeria
- Organizations
- Altura Credit Union, a credit union in California
- American Conservative Union, a political lobbying group in the United States
- Arab Customs Union, an organization under the Arab League for a Customs Union between Arab Members of the League
- Asian Clearing Union, an organization that settles international payments between member countries
- Assiniboine Credit Union, a credit union in Manitoba
- Acu Publishing, an imprint of the German group VDM Publishing devoted to the reproduction of Wikipedia content
- Military
- Assault Craft Unit, military units of the United States Navy that specialize in amphibious warfare
- Armoured Command Unit, the key unit in the RTS game Supreme Commander
- Army Combat Uniform, the combat uniform worn by the United States Army
- Transportation related
- Achutupo Airport, an airport in Achutupo, Panama with the IATA airport code "ACU"
- Auto-Cycle Union, an officially recognised motorcycle governing body of Great Britain
- Automovil Club del Uruguay, a member of the Fédération Internationale de l'Automobile
- Geography
- Açu, municipality in the state of Rio Grande do Norte, Brazil
- Açu River, another name for the Piranhas River in Brazil
- Pariquera-Açu, a municipality in the state of São Paulo in Brazil
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