Assembler (meaning one that assembles) may refer to:
- the assembly language, a computer program to translate between lower-level representations of computer programs; it converts basic computer instructions into a pattern of bits which can be easily understood by a computer and the processor can use it to perform its basic operations
- the assembler (bioinformatics), a program to perform genome assembly
- the assembler (nanotechnology), a conjectured construction machine that would manipulate and build with individual atoms or molecules
- the stage name of avant-garde electronic musician Nobukazu Takemura
- the Assemblers, an alien race in the Star Wars universe
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