Ayanami may refer to:
In Japanese naval destroyers:
- Japanese destroyer Ayanami (1909), World War I-era Kamikaze class destroyer
- Japanese destroyer Ayanami (1929), World War II-era Fubuki class destroyer
- Japanese destroyer Ayanami (DD-103), lead ship of the Japanese Maritime Self-Defense Force's Ayanami class destroyer
In others:
- Rei Ayanami, a character in the anime show Neon Genesis Evangelion
- Ayanami, a character dubbed by Hayami Shou in the anime show 07-Ghost
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