Barleycorn may mean:
- a grain of barley
- English unit of length equal to 1/3 inch
- The Barleycorn, an Irish music group
- "John Barleycorn", an ancient folksong
- Edward Thaddeus Barleycorn Barber
- William Barleycorn, a Primitive Methodist missionary in Fernando Po
- John Barleycorn (novel) by writer Jack London
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