Bassein may refer to:
- Pathein, the capital city of Ayeyarwady Division, Myanmar (Burma); formerly known as Bassein.
- Vasai, a city in Thane District, Maharashtra, India; known as Bassein during Portuguese rule.
- Bassein Fort, a fort in Vasai (old name Bassein), built by the Portuguese.
- Bassein River (see Pathein River), a distributary of the Yangon River in Burma.