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- Cue stick, in billiard-type games
- Cue bid, a type of bid in the card game contract bridge
- "Cue" (among other spellings), a spelled-out name for the letter Q in the English alphabet
- ".cue", used in the filename of cue sheets, descriptor files for specifying the layout of CD or DVD tracks
- CUE, commercially useful enzymes
- CUE, an infotainment, navigation and communication system used in sedans by Cadillac
- CUE Bus (City–University–Energysaver), the local bus system operated by the city of Fairfax, Virginia, United States
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