In Entertainment
- Darwin (film), a 2011 documentary film
- Darwin (seaQuest DSV), a dolphin in the TV series seaQuest DSV
- Darwin 4078, an arcade game
- Darwin (comics), a fictional character in the Marvel Comics Universe, associated with X-Men
- Darwin!, a 1972 musical album by Banco del Mutuo Soccorso
- Darwin's Nightmare, a 2004 documentary film
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