DUT is an acronym for
- Da Nang University of Technology in Da Nang, Viet Nam
- Dalian University of Technology in Dalian, China
- Delft University of Technology in Delft, the Netherlands
- Device under test, especially in semiconductor testing
- Diplôme universitaire de technologie, a French undergraduate university degree in technology
- Durban University of Technology in Durban, South Africa
- DUT (gene), which encodes DUTP pyrophosphatase
- DUT1 or DUT, which describes the difference between coordinated universal time (UTC) and universal time (UT)
- Unalaska Airport (IATA airport code "DUT"), an airport in Unalaska, Alaska, U.S.