FLI may stand for:
- Flixton railway station on the Manchester/Liverpool line in England
- Friedrich Loeffler Institute, a research centre for animal health in Greifswald in Germany
- Future and Freedom (Futuro e Libertà per l'Italia), Italian political grouping
- Flexible Line Interpreter, graphic mode of MOS Technology VIC-II
- .fli, an extension for the FLIC animation file format
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