Folio may refer to:
- Folio (printing), a book size
- A particular edition of a book printed on folio pages, such as the First Folio of William Shakespeare's plays
- A leaf of a book: see Recto and verso
- Folio (typeface), a sans-serif typeface
- Folio (magazine)
- Foolscap folio, a paper size
- Folio Society, publishers of fine illustrated books
- Folio Weekly, a newspaper published in Florida and Georgia
- "Folio", an imprint of French publisher Éditions Gallimard
- Folio Corporation, a software company and technology product
- Folio, Inc. (acquired by Sun Microsystems), developers of the F3 font format
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