GLI may refer to:
- The Volkswagen GLI, a high performance version of the Volkswagen Jetta
- Great Lakes Initiative, the fictional Marvel Comics superteam formerly known as the Great Lakes Avengers
- Great Lakes Invitational, a college ice-hockey tournament
- Greyhound Lines, a North-American bus carrier
- IATA Airport Code for Glen Innes Airport, Australia.
- Getty Leadership Institute at Claremont Graduate University
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