Harkonnen may refer to:
- House Harkonnen, the fictional noble family in the Dune series of novels and films
- Dune: House Harkonnen, the second book in the Prelude to Dune series, written by Brian Herbert and Kevin J. Anderson
- Harkonnen, a weapon used by Seras Victoria in the series Hellsing. The weapons's full name is the Hellsing ARMS Anti-Midian Cannon 'Harkonnen'.
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