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- Mayonnaise, sometimes abbreviated to mayo
- Mayo Clinic, a medical center in Rochester, Minnesota, United States
- Mayo Medical School, an American medical school that is part of the Mayo Clinic and the Mayo College of Medicine
- Mayo High School, a public high school in Rochester, Minnesota, United States
- Mayo College, a secondary educational institution in Ajmer, Rajasthan, India
- Mayo GAA, an inter-county Gaelic Athletic Association team
- Mayo people, an Indigenous ethnic group who live in the Mexican states of Sinaloa and Sonora
- Mayo Rajputs or Meo Rajputs, an Indian ethnic tribe of Rajputs also spelled as meo
- Mayo language, spoken by the Mayo people
- Mayo (TV series), a BBC television series first broadcast in 2006
- Earl of Mayo, a title in the Peerage of Ireland
- Mayo Hospital, in Lahore, Pakistan
- The Mexican American Youth Organization
- Project Mayo, an open source project by DivX, Inc.
- Mayo, the Spanish word for May
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