MDF may refer to:
- Market development funds
- Maryland Deathfest
- Moksha language
- Medium-density fibreboard, a type of particle board made of small particles of wood
- Modulation transfer function, the optical transfer function magnitude with phase ignored
In computers:
- Max Disk Formatter
- MES Development Framework, a .NET framework for building Manufacturing execution system applications
- Message Development Framework, a collection of models, methods and tools used by Health Level 7 v3.0 methodology
- Master Database File, a file extension for Microsoft SQL Server.
- Measurement Data Format, a file format used by the automotive industry for storing measurement data. it contains raw measurement data from a measurement and the metadata necessary to interpret the raw data.
In medicine:
- Map-dot-fingerprint dystrophy, a genetic disease affecting the cornea
- Mean Diastolic Filling of the heart
- Myocardial depressant factor, a low-molecular-weight peptide released from the pancreas into the blood in mammals during various shock states
In organizations:
- Hungarian Democratic Forum (Hungarian: Magyar Demokrata Fórum), a political party
- Mymensingh Debate Federation, a Bangladeshi debate organization
- Myotonic Dystrophy Foundation, a U.S. non-profit organization related to myotonic dystrophy
- Marché du Film, professional film market at the Cannes Film Festival
In telecommunication:
- Main distribution frame, a distribution frame where cables are cross-connected in telephony
- Multidelay block frequency domain adaptive filter