Sequencing - Protein Sequencing

Protein Sequencing

Methods for performing protein sequencing include:

  • Edman degradation
  • Peptide mass fingerprinting
  • Mass spectrometry
  • Protease digests

If the gene encoding the protein can be identified it is currently much easier to sequence the DNA and infer the protein sequence. Determining part of a protein's amino-acid sequence (often one end) by one of the above methods may be sufficient to enable the identification of a clone carrying the gene.

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