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- University of Toledo, a public research university located in Toledo, Ohio, United States
- Toledo Rockets, the athletic program of the University of Toledo
- Toledo, a version of the AMD Athlon 64 X2 CPU
- Toledo (K.U. Leuven), a project at the Katholieke Universiteit Leuven
- "Toledo", a song by Elvis Costello and Burt Bacharach for the 1998 Painted from Memory album
- Toledo Colônia Work, a Brazilian football (soccer) club
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